Comparative Matrix for Menstrual Stromal Cells and Bone Marrow Mesenchymal
Cells
Cell Characteristics |
C’elle (Menstrual
Stromal Cells) |
BM MSC (Bone Marrow
Mesenchymal Cells) |
Cell Collection Procedure |
Non-invasive menstrual cup similar to insertion
of a tampon |
Harvest bone marrow in the OR mobilized adult
peripheral blood |
Cell Morphology |
stromal, mesenchymal-like, spindle-shaped
in culture |
stromal, mesenchymal-like, spindle-shaped
in culture (4) |
Cell Surface Markers associated with mesenchymal
cells |
CD29, CD44, CD73, CD90, CD105, CD166
MHC I+ MHC II- |
CD29, CD44, CD73, CD90, CD105, CD166
MHC I+ MHC II- (1)(3) |
Markers associated with embryonic stem cells |
SSEA-4, Oct-4 |
none |
Differentiation capability |
Neural, Cardiogenic, Chondrogenic, Adipogenic,
Osteogenic |
Chondrogenic, Adipogenic, Osteogenic (2) |
MLR |
Cells demonstarted a weak stimulatory response
which suggests potential use in first-degree relatives |
Do not elicit alloreactive lymphocyte proliferative
responses (1) |
Karotype |
Normal karyotype |
Normal Karyotye (3) |
CD 117 positive |
Yes |
Yes (7,8,9) |
References:
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K, Zetterberg
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- Pittenger
MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, et al. (1999). Multilineage
potential of adult human mesenchymal stem cells. Science, 284(5411),
143-147.
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Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood,
or Adipose Tissue. Stem Cells, 24(5), 1294 –1301.
- Horwitz, Edwin M. (2007). Report on the Workshop "New Technologies
in Stem Cell Research," Society for Pediatric Research, San Francisco,
California, April 29, 2006, Fundamentals of MSC Isolation and Purification.Stem
Cells, 25(4), 1070 -1088.
- Schachinger V, Erbs S, Elsasser A, Haberbosch W, Hambrecht R, et al. (2006).
Intracoronary bone marrow-derived progenitor cells in acute myocardial infarction. N
Engl J Med, 355(12), 1210-1221.
- Horwitz EM, Prockop DJ, Fitzpatrick LA, Koo WW, Gordon PL, et al. (1999).
Transplantability and therapeutic effects of bone marrow-derived mesenchymal
cells in children with osteogenesis imperfecta. Nat Med, 5, 309–313.
- Gentry, T; Foster, SJ.; Winstead, L; Deibert, E; Fiordalisi, M; Balber, AE. Simultaneous isolation of human bone marrow hematopoietic, endothelial, and mesenchymal progenitor cells by flow sorting based on ahlehyde dehydrogenase activity. (2006) Blood (108;11, part 1), 1043A
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